Skip to main content
profile image
Selected Works of Geoffrey "Geoff" Lin-Cereghino
Article
Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins
Biotechnology Letters
  • Sasha Larsen, University of the Pacific
  • Jun Weaver, University of the Pacific
  • Katherine de Sa Campos, University of the Pacific
  • Rhobe Bulahan, University of the Pacific
  • Jackson Nguyen, University of the Pacific
  • Heather Grove, University of the Pacific
  • Amy Huang, University of the Pacific
  • Lauren Low, University of the Pacific
  • Namphuong Tran, University of the Pacific
  • Seth Gomez, University of the Pacific
  • Jennifer Yau, University of the Pacific
  • Thomas Ilustrisimo, University of the Pacific
  • Jessica Kawilarang, University of the Pacific
  • Jonathan Lau, University of the Pacific
  • Maivi Tranphung, University of the Pacific
  • Irene Chen, University of the Pacific
  • Christina Tran, University of the Pacific
  • Marcia Fox, University of the Pacific
  • Joan Lin-Cereghino, University of the Pacific
  • Geoff P. Lin-Cereghino, University of the Pacific
Link
Document Type
Article
Department
Biological Sciences
DOI
10.1007/s10529-013-1290-7
Publication Date
11-1-2013
Disciplines
Abstract

Although Pichia pastoris is a popular protein expression system, it exhibits limitations in its ability to secrete heterologous proteins. Therefore, a REMI (restriction enzyme mediated insertion) strategy was utilized to select mutant beta-g alactosidase s upersecretion (bgs) strains that secreted increased levels of a β-galactosidase reporter. Many of the twelve BGS genes may have functions in intracellular signaling or vesicle transport. Several of these strains also appeared to contain a more permeable cell wall. Preliminary characterization of four bgs mutants showed that they differed in the ability to enhance the export of other reporter proteins. bgs13, which has a disruption in a gene homologous to Saccharomyces cerevisiae protein kinase C (PKC1), gave enhanced secretion of most recombinant proteins that were tested, raising the possibility that it has the universal super-secreter phenotype needed in an industrial production strain of P. pastoris.

Citation Information
Sasha Larsen, Jun Weaver, Katherine de Sa Campos, Rhobe Bulahan, et al.. "Mutant strains of Pichia pastoris with enhanced secretion of recombinant proteins" Biotechnology Letters Vol. 35 Iss. 11 (2013) p. 1925 - 1935 ISSN: 0141-5492
Available at: http://works.bepress.com/geoff-lin-cereghino/23/