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Selected Works of Geoffrey "Geoff" Lin-Cereghino
Article
Direct selection of Pichia pastoris expression strains using new G418 resistance vectors
Yeast
  • Joan Lin-Cereghino, University of the Pacific
  • Matthew Hashimoto, University of the Pacific
  • Allison Moy, University of the Pacific
  • James Castelo, University of the Pacific
  • Claire Orazem, University of the Pacific
  • Peter Kuo, University of the Pacific
  • See Xiong, University of the Pacific
  • Vishal Gandhi, University of the Pacific
  • Christopher Hatae, University of the Pacific
  • Alex Chan, University of the Pacific
  • Geoff P. Lin-Cereghino, University of the Pacific
Link
Document Type
Article
Department
Biological Sciences
DOI
10.1002/yea.1587
Publication Date
4-1-2008
Disciplines
Abstract

The methylotrophic yeast, Pichia pastoris, is widely used as a host organism for the expression of heterologous proteins. Currently, the Zeocin and blasticidin resistance genes are the only dominant selectable markers that can be used for primary selection of transformants. In this report we describe new expression vectors that can be used to select directly for P. pastoris transformants using G418 resistance conferred by a modified Tn903kanr gene. Compared to other dominant markers, this system is more economical and offers a higher transformation efficiency, due to the small sizes of the cloning vectors, pKAN B and pKANα B (GenBank Accession Nos EU285585 and EU285586, respectively). Additionally, multicopy transformants can be generated using these new vectors.

Citation Information
Joan Lin-Cereghino, Matthew Hashimoto, Allison Moy, James Castelo, et al.. "Direct selection of Pichia pastoris expression strains using new G418 resistance vectors" Yeast Vol. 25 Iss. 4 (2008) p. 293 - 299 ISSN: 0749-503X
Available at: http://works.bepress.com/geoff-lin-cereghino/13/