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A role for the Parkinson's disease protein DJ-1 as a chaperone and antioxidant in the anhydrobiotic nematode Panagrolaimus superbus
Cell Stress and Chaperones (2014)
  • Bridget A. Culleton, Dr
  • Patrick Lall
  • Gemma K Kinsella, Dr, National University of Ireland, Maynooth
  • Sean Doyle, Prof, National University of Ireland, Maynooth
  • John McCaffrey, Dr, National University of Ireland, Maynooth
  • David A Fitzpatrick, Dr, National University of Ireland, Maynooth
  • Ann M Burnell, Prof, National University of Ireland, Maynooth
Abstract

Mutations in the human DJ-1/PARK7 gene are associated with familial Parkinson’s disease. DJ-1 belongs to a large, functionally diverse family with homologues in all biological kingdoms. Several activities have been demonstrated for DJ-1: an antioxidant protein, a redox-regulated molecular chaperone and a modulator of multiple cellular signalling pathways. The majority of functional studies have focussed on human DJ-1 (hDJ-1), but studies on DJ-1 homologues in Drosophila melanogaster, Caenorhabditis elegans, Dugesia japonica and Escherichia coli also provide evidence of a role for DJ-1 as an antioxidant. Here, we show that dehydration is a potent inducer of a dj-1 gene in the anhydrobiotic nematode Panagrolaimus superbus. Our secondary structure and homology modelling analyses shows that recombinant DJ-1 protein from P. superbus (PsuDJ-1.1) is a well-folded protein, which is similar in structure to the hDJ-1. PsuDJ-1.1 is a heat stable protein; with T1/2 unfolding transition values of 76 and 70 °C obtained from both circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR) measurements respectively. We found that PsuDJ-1.1 is an efficient antioxidant that also functions as a ‘holdase’ molecular chaperone that can maintain its chaperone function in a reducing environment. In addition to its chaperone activity, PsuDJ-1.1 may also be an important non-enzymatic antioxidant, capable of providing protection to P. superbus from oxidative damage when the nematodes are in a desiccated, anhydrobiotic state.

Keywords
  • DJ-1,
  • Chaperone,
  • Antioxidant,
  • Anhydrobiosis,
  • Homology modelling,
  • Molecular dynamics
Disciplines
Publication Date
Fall October 16, 2014
Citation Information
Bridget A. Culleton, Patrick Lall, Gemma K Kinsella, Sean Doyle, et al.. "A role for the Parkinson's disease protein DJ-1 as a chaperone and antioxidant in the anhydrobiotic nematode Panagrolaimus superbus" Cell Stress and Chaperones Vol. 20 Iss. 1 (2014)
Available at: http://works.bepress.com/gemma_kinsella/2/