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Detection of Nitrate/Nitrite Bioavailability in Wastewater Using a luxCDABEBased Klebsiella oxytoca Bioluminescent Bioreporter
Journal of Microbiology and Biotechnology (2007)
  • Gary S. Sayler
  • Steven Ripp, University of Tennessee - Knoxville
  • Desoukky Abd-ElHaleem
  • Sahar Zaki
Abstract

In the present study, we have constructed a bioluminescent bioreporter for the assessment of nitrate/nitrite bioavailability in wastewater. Specifically, an approximately 500-bp DNA fragment containing a nitrate/nitrite-activated nasR-like promoter (regulating expression of genes encoding nitrite reductase in the genus Klebsiella) was fused upstream of the Vibrio fischeri luxCDABE gene cassette in a modified mini-Tn5 vector. Characterization of this strain, designated W6-1, yielded dose-dependent increased bioluminescence coincident with increased nitrate, nitrite, and ammonium added to the growth medium from 1 to 11 ppm. Bioluminescence in response to nitrogen species addition was light dependent up to 10, 7, and 8 ppm with nitrate, nitrite, and ammonium, respectively. This response was linear in the range from 1 to 8 ppm for nitrate (), 1 to 6 ppm for nitrite (), and 1 to 7 ppm for ammonium (). A significant bioluminescent response was also recorded when strain W6-1 was incubated with slurries from aged, nitrate/nitrite contaminated wastewater. Thus, bioreporter strain W6-1 can be used to elucidate factors that constrain the use of nitrate/nitrite in wastewaters.

Disciplines
Publication Date
2007
Citation Information
Gary S. Sayler, Steven Ripp, Desoukky Abd-ElHaleem and Sahar Zaki. "Detection of Nitrate/Nitrite Bioavailability in Wastewater Using a luxCDABEBased Klebsiella oxytoca Bioluminescent Bioreporter" Journal of Microbiology and Biotechnology Vol. 17 Iss. 8 (2007)
Available at: http://works.bepress.com/gary_sayler/9/