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Screening of potentially hormonally active chemicals using bioluminescent yeast bioreporters
Toxicological Sciences (2009)
  • John Sanseverino
  • Melanie L. Eldridge
  • Alice C. Layton
  • James P. Easter
  • Jason Yarbrough
  • Terry Wayne Schultz
  • Gary S. Sayler, University of Tennessee, Knoxville
Abstract
Saccharomyces cerevisiae bioluminescent bioreporter assays were developed previously to assess a chemical's estrogenic or androgenic disrupting potential. S. cerevisiae BLYES, S. cerevisiae BLYAS, S. cerevisiae BLYR, were used to assess their reproducibility and utility in screening 68, 69, and 71 chemicals for estrogenic, androgenic, and toxic effects, respectively. EC50 values were 6.3 ± 2.4 × 10−10M (n = 18) and 1.1 ± 0.5 × 10−8M (n = 13) for BLYES and BLYAS, using 17β-estradiol and 5α-dihydrotestosterone over concentration ranges of 2.5 × 10−12 through 1.0 × 10−6M, respectively. Based on analysis of replicate standard curves and comparison to background controls, a set of quantitative rules have been formulated to interpret data and determine if a chemical is potentially hormonally active, toxic, both, or neither. The results demonstrated that these assays are applicable for Tier I chemical screening in Environmental Protection Agency's Endocrine Disruptor Screening and Testing Program as well as for monitoring endocrine-disrupting activity of unknown chemicals in water. doi: 10.1093/toxsci/kfn229
Disciplines
Publication Date
2009
Citation Information
John Sanseverino, Melanie L. Eldridge, Alice C. Layton, James P. Easter, et al.. "Screening of potentially hormonally active chemicals using bioluminescent yeast bioreporters" Toxicological Sciences Vol. 107 Iss. 1 (2009)
Available at: http://works.bepress.com/gary_sayler/59/