Skip to main content
Article
Ameliorating risk: Culturable and metagenomic monitoring of the 14 year decline of a genetically engineered microorganism at a bioremediation field site
Journal of Bioremediation & Biodegradation (2012)
  • A. C. Layton
  • A. E. Smartt
  • A. Chauhan
  • S. Ripp
  • D. E. Williams
  • W. Burton
  • S. Moser
  • J. Phillips
  • A. V. Palumbo
  • Gary S. Sayler, University of Tennessee, Knoxville
Abstract
In 1996, the first EPA sanctioned release of a recombinant microbe (Pseudomonas fluorescens HK44) into the subsurface soil environment was initiated in a replicated semi-contained array of soil lysimeters. With an aim to access the survivability/environmental fate of HK44, soil sampling was performed 14 years post release. Although after extensive sampling culturable HK44 cells were not found, qPCR and metagenomic analyses indicated that genetic signatures of HK44 cells still persisted in the soils, with genes diagnostic for the bioluminescent transposon carried by strain HK44 (luxA and tetA) being found at low concentrations (< 5000 copies/g). Additionally, metagenome analysis of lysimeter 2 using amplicon pyrosequencing showed that Burkholderia was more abundant in the sample extracted before storage at 4°C than after storage at 4°C (79% and 5.6% Burkholderia sequences, respectively). http://www.omicsonline.org/2155-6199/2155-6199-S1-009.php?aid=5830 doi:10.4172/2155-6199.S1-009
Disciplines
Publication Date
2012
Citation Information
A. C. Layton, A. E. Smartt, A. Chauhan, S. Ripp, et al.. "Ameliorating risk: Culturable and metagenomic monitoring of the 14 year decline of a genetically engineered microorganism at a bioremediation field site" Journal of Bioremediation & Biodegradation Vol. S1 Iss. 009 (2012)
Available at: http://works.bepress.com/gary_sayler/31/