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Article
Transcriptome profiling of soybean (Glycine max) roots challenged with pathogenic and non-pathogenic isolates of Fusarium oxysporum
BMC Genomics
  • Alessandra Lanubile, Iowa State University
  • Usha K. Muppirala, Iowa State University
  • Andrew J. Severin, Iowa State University
  • Adriano Marocco, Università Cattolica del Sacro Cuore
  • Gary P. Munkvold, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
12-21-2015
DOI
10.1186/s12864-015-2318-2
Abstract
Background Fusarium oxysporum is one of the most common fungal pathogens causing soybean root rot and seedling blight in U.S.A. In a recent study, significant variation in aggressiveness was observed among isolates of F. oxysporum collected from roots in Iowa, ranging from highly pathogenic to weakly or non-pathogenic isolates. Results We used RNA-seq analysis to investigate the molecular aspects of the interactions of a partially resistant soybean genotype with non-pathogenic/pathogenic isolates of F. oxysporum at 72 and 96 h post inoculation (hpi). Markedly different gene expression profiles were observed in response to the two isolates. A peak of highly differentially expressed genes (HDEGs) was triggered at 72 hpi in soybean roots and the number of HDEGs was about eight times higher in response to the pathogenic isolate compared to the non-pathogenic one (1,659 vs. 203 HDEGs, respectively). Furthermore, the magnitude of induction was much greater in response to the pathogenic isolate. This response included a stronger activation of defense-related genes, transcription factors, and genes involved in ethylene biosynthesis, secondary and sugar metabolism. Conclusions The obtained data provide an important insight into the transcriptional responses of soybean-F. oxysporum interactions and illustrate the more drastic changes in the host transcriptome in response to the pathogenic isolate. These results may be useful in the developing new methods of broadening resistance of soybean to F. oxysporum, including the over-expression of key soybean genes.
Comments

This article is from BMC Genomics 16 (2015): 1089, doi:10.1186/s12864-015-2318-2. Posted with permission.

Rights
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright Owner
Lanubile et al.
Language
en
File Format
application/pdf
Citation Information
Alessandra Lanubile, Usha K. Muppirala, Andrew J. Severin, Adriano Marocco, et al.. "Transcriptome profiling of soybean (Glycine max) roots challenged with pathogenic and non-pathogenic isolates of Fusarium oxysporum" BMC Genomics Vol. 16 (2015) p. 1089
Available at: http://works.bepress.com/gary_munkvold/130/