Article
Exercise Training, Indomethacin, and Isoproterenol-Induced Myocardial Necrosis in the Rat
Basic Research in Cardiology
(1991)
Abstract
It was hypothesized that endurance exercise training would attenuate isoproterenol-induced myocardial necrosis in the rat by increasing the concentration of prostacyclin in the myocardial vasculature. Rats were randomly assigned to exercise and control groups. Exercisers ran on a motorized treadmill 1 h · d−1, 5 d · week−1 for 14 weeks. Immediately following the training program subgroups of rats were injected with 4 mg · kg−1 indomethacin or saline. One day later, all rats were given a subcutaneous injection of isoproterenol (20 mg · kg−1); after another 24h they were sacrificed. A decrease of myocardial creatine kinase (CK) activity was used as a marker for myocardial necrosis. Endurance exercise training attenuated the isoproterenol-induced decrease in myocardial CK relative to control by approximately 37% (exercise: 16.4 ± 0.6 U · mg−1 protein; control: 10.5 ± 0.6 U · mg−1 protein; p < 0.05). Pretreatment with indomethacin decreased myocardial CK in the exercise-trained rats (indomethacin: 15.4 ± 0.8 U · mg−1 protein; saline: 17.7 ± 0.7 U · mg−1 protein; p < 0.05), but not in the controls (indomethacin: 10.3 ± 1.0 U · mg−1 protein; saline: 10.8 ± 0.6 U · mg−1 protein; p > 0.05). The concentration of myocardial 6-keto-PGF1α a marker for prostacyclin, was not altered by exercise but, as expected, was reduced by indomethacin pretreatment (p < 0.05). Thus, exercise training reduces myocardial damage caused by isoproterenol, but the evidence does not support the hypothesis that prostacyclin mediated this effect of training. Further research is needed to determine the extent to which exercise training-induced alterations in sensitivity to PGI2 or TXA2 affect myocardial damage from isoproterenol. The effect of any exercise-induced alteration in cardiac β-adrenoceptor number or binding characteristics which result in myocardial resistance to isoproterenol also cannot be overlooked.
Disciplines
Publication Date
January, 1991
Citation Information
Gary Brodowicz and D. R. Lamb. "Exercise Training, Indomethacin, and Isoproterenol-Induced Myocardial Necrosis in the Rat" Basic Research in Cardiology Vol. 86 Iss. 1 (1991) Available at: http://works.bepress.com/gary_brodowicz/14/