Body composition of both younger and older subjects was estimated using several different methods in order to evaluate their use with elderly subjects. Estimates were obtained by dual energy x-ray absorptiometry, underwater weighing, bioelectrical impedance analysis, and skinfold measurement in 48 younger subjects (26–40 years) and 44 older subjects (65–85 years). In older men and women the underwater weighing percent fat estimates were significantly higher than all other methods. Bone mineral explained a significant proportion of the variance in the difference between the dual energy x-ray absorptiometry and underwater weighing estimates of percent fat (R2 = 0.442-0.627). Because of its insensitivity to variability in bone mineral, we recommend that the underwater weighing method be not used to estimate percent fat in older men and women. Dual energy x-ray absorptiometry provides an alternative which accounts for the age-related decrease in bone mineral density.