A chicken genomic library was screened for novel homeobox-like elements. We describe a chicken genomic DNA fragment which hybridizes to the murine Hox-3.1 homeobox. The fragment is a single-copy sequence which seems to be transcribed without spacial or temporal restrictions. The sequence presented here is not representative of anything yet in the databases; however, it contains a very probable open reading frame which would encode a peptide slightly homologous to the Hox-3.1 protein.