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Selected Works of Gary S. Goldberg
Article
A preloading method of evaluating gap junctional communication by fluorescent dye transfer
BioTechniques (1995)
  • Gary Goldberg, Rowan University School of Osteopathic Medicine
  • John F. Bechberger, The University of Western Ontario
  • C.G. Naus
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Abstract
We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and DiI. Calcein is able to pass through gap junctions, while DiI is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.

Note: Erratum in Biotechniques 1995 Aug;19(2):212.
Keywords
  • Carbenoxolone,
  • Carbocyanines,
  • Cell Communication,
  • Connexin 43,
  • Fluoresceins,
  • Metabolism,
  • Fluorescent Dyes,
  • Gap Junctions,
  • Glycyrrhetinic Acid,
  • Oncogene Protein pp60(v-src),
  • Stereoisomerism,
  • Succinates,
  • Pharmacology,
  • 3,
  • 3'-dioctadecylindocarbocyanine,
  • Fluorexon
Disciplines
Publication Date
March, 1995
Citation Information
Gary Goldberg, John F. Bechberger and C.G. Naus. "A preloading method of evaluating gap junctional communication by fluorescent dye transfer" BioTechniques Vol. 18 Iss. 3 (1995) p. 490 - 497 ISSN: print: 0736-6205 online: 1940-9818
Available at: http://works.bepress.com/gary-s-goldberg/43/