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Bacillus nakamurai sp. nov., a black-pigment-producing strain
International Journal of Systematic and Evolutionary Microbiology (2016)
  • Christopher A Dunlap
  • Lauren P Saunders
  • David A Schisler
  • Timothy D Leathers
  • Naveed Naeem
  • Frederick M Cohan
  • Alejandro P Rooney
Abstract
Two isolates of a Gram-stain-positive, strictly aerobic, motile, rod-shaped, endospore-forming bacterium were identified during a survey of the Bacillus diversity of the Agriculture Research Service Culture Collection. These strains were originally isolated from soil and have a phenotype of producing a dark pigment on tryptic soy agar. Phylogenetic analysis of the 16S rRNA gene indicated that these strains were related most closely to Bacillus subtilis subsp. inaquosorum (99.7 % similarity) and Bacillus axarquiensis (99.7 %). In phenotypic characterization, the novel strains were found to grow between 17 and 50 °C and can tolerate up to 9 % (w/v) NaCl. Furthermore, the strains grew in media of pH 5.5–10 (optimal growth at pH 7.0–8.0). The predominant cellular fatty acids were anteiso-C15 : 0 (34.8 %) and iso-C15 : 0 (21.9 %). The cell-wall peptidoglycan contained meso-diaminopimelic acid. A draft genome of both strains was completed. The DNA G+C content was 43.8 mol%. A phylogenomic analysis on the core genome of these two new strains and all members of the Bacillus subtilis group revealed these two strains formed a distinct monophyletic clade with the nearest neighbour Bacillus amyloliquefaciens . DNA–DNA relatedness studies using in silico DNA–DNA hybridizations showed the two strains were conspecific (93.8 %), while values with all other species (<31.5 %) were well below the species threshold of 70 %. Based on the consensus of phylogenetic and phenotypic analyses, these strains are considered to represent a novel species within the genus Bacillus , for which the name Bacillus nakamurai sp. nov. is proposed, with type strain NRRL B-41091T (=CCUG 68786T).
Keywords
  • black,
  • pigment
Publication Date
2016
DOI
10.1099/ijsem.0.001135
Citation Information
Christopher A Dunlap, Lauren P Saunders, David A Schisler, Timothy D Leathers, et al.. "Bacillus nakamurai sp. nov., a black-pigment-producing strain" International Journal of Systematic and Evolutionary Microbiology Vol. 66 Iss. 8 (2016) p. 2987 - 2991
Available at: http://works.bepress.com/frederick_cohan/77/