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Article
Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution
Acta Crystallographica Section F, Structural Biology and Crystallization Communications
  • Laura-Lee Clancy Kelley
  • Bret D. Dillard
  • Wolfram Tempel
  • Lirong Chen
  • Neil Shaw
  • Doowon Lee
  • M. Gary Newton
  • Frank J. Sugar
  • Francis E. Jenney, Philadelphia College of Osteopathic Medicine
  • Han Seung Lee
  • Claudia Shah
  • Farris L. Poole
  • Michael W. W. Adams
  • Jane S. Richardson
  • David C. Richardson
  • Zhi-Jie Liu
  • Bi-Cheng Wang
  • John Rose
Document Type
Article
Publication Date
7-1-2007
Abstract
The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.
PubMed ID
17620707
Comments

This article was published in Acta Crystallographica Section F, Structural Biology and Crystallization Communications. Volume 63, Pt 7, July 2007, Pages 549-552.

The published version is available at http://dx.doi.org/10.1107/S1744309107024049

Copyright © 2007 International Union of Crystallography

Citation Information
Laura-Lee Clancy Kelley, Bret D. Dillard, Wolfram Tempel, Lirong Chen, et al.. "Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution" Acta Crystallographica Section F, Structural Biology and Crystallization Communications Vol. 63 Iss. Pt 7 (2007) p. 549 - 552
Available at: http://works.bepress.com/francis_jenney/75/