Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A ResolutionActa Crystallographica Section F, Structural Biology and Crystallization Communications
AbstractThe hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.
Citation InformationLaura-Lee Clancy Kelley, Bret D. Dillard, Wolfram Tempel, Lirong Chen, et al.. "Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution" Acta Crystallographica Section F, Structural Biology and Crystallization Communications Vol. 63 Iss. Pt 7 (2007) p. 549 - 552
Available at: http://works.bepress.com/francis_jenney/75/