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A neutron crystallographic analysis of a rubredoxin mutant
Applied Physics A: Materials Science and Processing
  • T. Chatake
  • K. Kurihara
  • I. Tanaka
  • M. W. Adams
  • Francis E. Jenney, Jr., Philadelphia College of Osteopathic Medicine
  • I. Tsyba
  • R. Bau
  • N. Niimura
Document Type
Article
Publication Date
1-1-2002
Disciplines
Abstract
In order to study the unusual thermostability of rubredoxin from Pyrococcus furiosus (RubPf), the structure of a 'triple mutant' of this rubredoxin, which is less thermostable than the wild type, was solved at 1.5-Å resolution by neutron-diffraction analysis using the BIX-3 diffractometer at the JRR-3M reactor of JAERI. The positions of the non-hydrogen atoms are almost the same as the native rubredoxin; however, for Trp3 → Tyr3, large structural changes were found, so that their hydrogen-bonding schemes are significantly different. Some positions of the hydrogen atoms and molecules of hydration are shifted in certain regions, suggesting that such differences may contribute to the differences in thermostability between this 'triple-mutant' rubredoxin and wild-type rubredoxin.
Comments

This article was published in Applied Physics A: Materials Science and Processing, Volume 74, Issue SUPPL.II, Pages S1280-S1282.

The published version is available at http://dx.doi.org/10.1007/s003390201696.

Copyright © 2002 Springer.

Citation Information
T. Chatake, K. Kurihara, I. Tanaka, M. W. Adams, et al.. "A neutron crystallographic analysis of a rubredoxin mutant" Applied Physics A: Materials Science and Processing Vol. 74 Iss. SUPPL.II (2002) p. S1280 - S1282
Available at: http://works.bepress.com/francis_jenney/45/