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Sulfur K-edge X-ray absorption spectroscopy and density functional theory calculations on superoxide reductase: Role of the axial thiolate in reactivity
Journal of the American Chemical Society
  • A. Dey
  • Francis E. Jenney, Jr., Philadelphia College of Osteopathic Medicine
  • M. W. Adams
  • M. K. Johnson
  • K. O. Hodgson
  • B. Hedman
  • E. I. Solomon
Document Type
Article
Publication Date
1-1-2007
Disciplines
Abstract
Superoxide reductase (SOR) is a non-heme iron enzyme that reduces superoxide to peroxide at a diffusion-controlled rate. Sulfur K-edge X-ray absorption spectroscopy (XAS) is used to investigate the ground-state electronic structure of the resting high-spin and CN- bound low-spin Fe III forms of the 1Fe SOR from Pyrococcus furiosus. A computational model with constrained imidazole rings (necessary for reproducing spin states), H-bonding interaction to the thiolate (necessary for reproducing Fe-S bond covalency of the high-spin and low-spin forms), and H-bonding to the exchangeable axial ligand (necessary to reproduce the ground state of the low-spin form) was developed and then used to investigate the enzymatic reaction mechanism. Reaction of the resting ferrous site with superoxide and protonation leading to a high-spin FeIII-OOH species and its subsequent protonation resulting in H2O2 release is calculated to be the most energetically favorable reaction pathway. Our results suggest that the thiolate acts as a covalent anionic ligand. Replacing the thiolate with a neutral noncovalent ligand makes protonation very endothermic and greatly raises the reduction potential. The covalent nature of the thiolate weakens the FeIII bond to the proximal oxygen of this hydroperoxo species, which raises its pKa by an additional 5 log units relative to the pK a of a primarily anionic ligand, facilitating its protonation. A comparison with cytochrome P450 indicates that the stronger equatorial ligand field from the porphyrin results in a low-spin FeIII-OOH species that would not be capable of efficient H2O2 release due to a spin-crossing barrier associated with formation of a high-spin 5C Fe III product. Additionally, the presence of the dianionic porphyrin π ring in cytochrome P450 allows O-O heterolysis, forming an Fe IV-oxo porphyrin radical species, which is calculated to be extremely unfavorable for the non-heme SOR ligand environment. Finally, the 5C Fe III site that results from the product release at the end of the O2- reduction cycle is calculated to be capable of reacting with a second O2-, resulting in superoxide dismutase (SOD) activity. However, in contrast to FeSOD, the 5C FeIII site of SOR, which is more positively charged, is calculated to have a high affinity for binding a sixth anionic ligand, which would inhibit its SOD activity. © 2007 American Chemical Society.
Comments

This article was published in Journal of the American Chemical Society, Volume 129, Issue 41, Pages 12418-12431.

The published version is available at http://dx.doi.org/10.1021/ja064167p.

Copyright © 2007 ACS.

Citation Information
A. Dey, Francis E. Jenney, M. W. Adams, M. K. Johnson, et al.. "Sulfur K-edge X-ray absorption spectroscopy and density functional theory calculations on superoxide reductase: Role of the axial thiolate in reactivity" Journal of the American Chemical Society Vol. 129 Iss. 41 (2007) p. 12418 - 12431
Available at: http://works.bepress.com/francis_jenney/32/