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Article
Purification and characterization of Aspergillus niger α-L-rhamnosidase for the biotransformation of naringin to prunin
African Journal of Microbiology Research
  • Hui Ni, Jimei University
  • An Feng Xiao, Jimei University
  • Hui Nong Cai, Jimei University
  • Feng Chen, Clemson University
  • Qi You, Clemson University
  • Yun Zheng Lu, Jimei University
Document Type
Article
Publication Date
6-1-2012
Publisher
Academic Journals
Disciplines
Abstract
This study was conducted to increase the bioactivity of litchi pericarp polysaccharides (LPPs) biotransformed by Aspergillus awamori. Comparedtothenon-A. awamori-fermented LPP, the growth effects of A. awamori-fermented LPP on Lactobacillus bulgaricus and Streptococcus thermophilus were four and two times higher after 3 days of fermentation, respectively. Increased 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and DNA protection activity of litchi pericarp polysaccharides were also achieved after A. awamori fermentation. Moreover, the relative content of glucose and arabinose in LPP after fermentation decreased from 58.82% to 22.60% and from 18.82% to 10.09%, respectively, with a concomitant increase in the relative contents of galactose, rhamnose, xylose, and mannose. Furthermore, lower molecular weight polysaccharides were obtained after A. awamori fermentation. It can be concluded that A. awamori was effective in biotransforming LPP into a bioactive mixture with lower molecular weight polysaccharides and higher antioxidant activity and relative galactose content.
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