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A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry
Molecules
  • Jing Li, Chinese Academy of Sciences
  • Haihui Xie, Chinese Academy of Sciences
  • Bao Yang, Chinese Academy of Sciences
  • Xinhong Dong, Chinese Academy of Sciences
  • Linyan Feng, Chinese Academy of Sciences
  • Feng Chen, Clemson University
  • Yueming Jiang, Chinese Academy of Sciences
Document Type
Article
Publication Date
1-1-2010
Publisher
MDPI
Disciplines
Abstract
Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that OTA was not present in the longan pulp, indicating that the presence of OTA in longan fruit pulp detected by the HPLC-FD analysis needed to be confirmed by the ESI-MS method.
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