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Enhancement of trace organic contaminant degradation by crude enzyme extract from Trametes versicolor culture: Effect of mediator type and concentration
Faculty of Engineering and Information Sciences - Papers: Part A
  • Ngoc Luong Nguyen, University of Wollongong
  • Faisal Ibney Hai, University of Wollongong
  • Jinguo Kang, University of Wollongong
  • Frederic Leusch, Griffith University
  • Felicity Roddick, RMIT University
  • S Faraj Magram, King Abdulaziz University, Saudi Arabia
  • William Price, University of Wollongong
  • Long Nghiem, University of Wollongong
RIS ID
89833
Publication Date
1-1-2014
Publication Details

Nguyen, L., Hai, F. I., Kang, J., Leusch, F., Roddick, F., Magram, S. Faraj., Price, W. E. & Nghiem, L. D. (2014). Enhancement of trace organic contaminant degradation by crude enzyme extract from Trametes versicolor culture: Effect of mediator type and concentration. Journal of the Taiwan Institute of Chemical Engineers, 45 (4), 1855-1862.

Abstract
The performance of two redox mediating compounds, namely 1-hydroxybenzotriazole (HBT) and syringaldehyde (SA), was compared in terms of enhancement of enzymatic degradation of a diverse set of 14 phenolic and 16 non-phenolic trace organic contaminants (TrOCs) and the toxicity of the treated media. Extracellular enzyme extract (predominantly containing laccase) from Trametes versicolor culture achieved efficient degradation (70-95%) of nine phenolic and one non-phenolic TrOCs. Mediator dosing extended the spectrum of efficiently degraded TrOCs to 13 phenolic and three non-phenolic compounds, with moderate improvements in removal of a few other non-phenolic compounds. TrOC removal efficiency improved significantly as the HBT dose was increased from 0.1 to 0.5 mM, while SA achieved similar removal over dosage range of 0.1-1 mM. A particular concern was the toxicity of the treated media (1200-2200 times that of the control) for all SA dosages applied. Overall, HBT at a concentration of 0.5 mM achieved the best removal without raising concern regarding toxicity of the treated media. The results are discussed in the light of the redox potential of the enzyme-mediator cocktail, the balance between the stability and reactivity of the radicals generated and their cytotoxic effects.
Citation Information
Ngoc Luong Nguyen, Faisal Ibney Hai, Jinguo Kang, Frederic Leusch, et al.. "Enhancement of trace organic contaminant degradation by crude enzyme extract from Trametes versicolor culture: Effect of mediator type and concentration" (2014)
Available at: http://works.bepress.com/faisal_hai/53/