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Article
Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies
Journal of Clinical Laboratory Analysis
  • María Elena Marsón, Facultad de Ciencias Exactas, Universidad Nacional de La Plata
  • Diego Dante Dana, Universidad Nacional de La Plata
  • Jaime Altcheh, Hospital de Ninos Ricardo Gutierrez
  • Facundo García-Bournissen, Hospital de Ninos Ricardo Gutierrez
  • Guido Mastrantonio, Facultad de Ciencias Exactas, Universidad Nacional de La Plata
Document Type
Article
Publication Date
9-1-2013
URL with Digital Object Identifier
10.1002/jcla.21615
Disciplines
Abstract

Objective: Chagas disease constitutes a major public health problem in Latin America. Correctly designed pharmacokinetic, safety, and bioequivalence studies are desirable in order to fill the knowledge gaps that presently exist on available drugs. It is necessary to develop accurate, simple, reproducible, and sensitive high-performance liquid chromatography (HPLC)/UV methods for the quantization of benznidazole (BNZ) in human plasma and urine for clinical applications, specially in pediatric patients. Methods: Quantization of BNZ in human plasma involved freeze-drying and re-suspension in organic solvent followed by reverse phase HPLC with UV detection. Analysis of BNZ in urine involved liquid/liquid extraction followed by reverse phase HPLC with UV detection. Results: Limits of quantization (LOQ) were 0.32 μg/ml for plasma and 5.2 μg/ml for urine. No metabolite interferences were showed in both methods. Conclusion: The LOQ of methods seems appropriate in pediatric clinical contexts. Both procedures were applied with good results, to the quantization of BNZ in plasma and urine of patients treated for Chagas disease. © 2013 Wiley Periodicals, Inc.

Citation Information
María Elena Marsón, Diego Dante Dana, Jaime Altcheh, Facundo García-Bournissen, et al.. "Development of UV/HPLC methods for quantitative analysis of benznidazole in human plasma and urine for application in pediatric clinical studies" Journal of Clinical Laboratory Analysis Vol. 27 Iss. 5 (2013) p. 384 - 390
Available at: http://works.bepress.com/facundo-garcia-bournissen/15/