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Strategy for the biotransformation of fermented palm oil mill effluent into biodegradable polyhydroxyalkanoates by activated sludge
Chemical Engineering Journal (2015)
Abstract

Management of wastewater by resource recovery approach allows the transformation of wastewater into valuable resources. This work examines the biotransformation of fermented palm oil mill effluent (POME) into biodegradable plastics - polyhydroxyalkanoates (PHA) - through cultivation and enrichment of PHA-accumulating organisms in activated sludge and the subsequent production of PHA by the cultivated sludge. Enrichment of PHA-accumulating organisms via aerobic dynamic feeding process was effective and had significantly enhanced the PHA storage capacity of the sludge (wt% PHA per sludge dry weight) from 4 wt% (seed sludge) to 40-64 wt% (sludge cultivated for 50 days and more). The cultivated sludge comprised of 42 +/- 12% Betaproteobacteria, 35 +/- 7% Alphaproteobacteria, and 13 4% Gammaproteobacteria, as estimated by fluorescent in situ hybridization. The influence of pH (4.5 in the absence of pH control; and pH 7,8 and 9) on the production of PHA by the cultivated sludge was subsequently investigated. Neutral pH was the most favorable for PHA production, resulting in a PHA content of 64 wt% in 8 h. The PHA produced was made up of 77 mol% 3-hydroxybutyrate and 23 mol% 3-hydroxyvalerate. These findings signify that the combination of fermented POME and activated sludge offers an alternative to the palm oil and the plastics industries for a more sustainable POME management and an economical PHA production route. (C) 2015 Elsevier B.V. All rights reserved. Link to Full-Text Articles : http://www.sciencedirect.com/science/article/pii/S1385894715001400

Keywords
  • aerobic dynamic feeding,
  • feast and famine,
  • palm oil mill effluent,
  • polyhydroxyalkanoates,
  • resource recovery,
  • volatile fatty acids,
  • sequencing batch reactor,
  • municipal waste-water,
  • volatile fatty-acids,
  • sugar-cane molasses,
  • organic load rate,
  • mixed cultures,
  • pha production,
  • nile blue,
  • microbial community,
  • polymer production
Publication Date
June 1, 2015
Publisher Statement
Ce4kw Times Cited:0 Cited References Count:52
Citation Information
"Strategy for the biotransformation of fermented palm oil mill effluent into biodegradable polyhydroxyalkanoates by activated sludge" Chemical Engineering Journal Vol. 269 (2015)
Available at: http://works.bepress.com/facultyofengineering_universityofmalaya/6/