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Materials and methods for the alteration of enzyme and acetyl CoA levels in plants
Iowa State University Patents
  • Basil J. Nikolau, Iowa State University
  • Eve S. Wurtele, Iowa State University
  • David J. Oliver, Iowa State University
  • Patrick S. Schnable, Iowa State University
  • Tsui-Jung Wen, Iowa State University
Document Type
Patent
Publication Date
4-28-2009
Abstract
The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL),Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
Patent Number
US 7,524,678 B2
Assignee
Iowa State University Research Foundation, Inc.
Application Number
11/167,856
Date Filed
6-27-2005
Language
en
File Format
application/pdf
Citation Information
Basil J. Nikolau, Eve S. Wurtele, David J. Oliver, Patrick S. Schnable, et al.. "Materials and methods for the alteration of enzyme and acetyl CoA levels in plants" (2009)
Available at: http://works.bepress.com/eve-wurtele/9/