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Article
In vitro and in silico annotation of conserved and nonconserved microRNAs in the genome of the marsupial Monodelphis domestica
The Journal of heredity
  • Eric J. Devor, University of Iowa
  • P. B. Samollow
Document Type
Article
Peer Reviewed
1
Publication Date
1-1-2008
NLM Title Abbreviation
J Hered
PubMed ID
17965199
DOI of Published Version
10.1093/jhered/esm085
Abstract
The gray short-tailed opossum, Monodelphis domestica, is the world's most widely utilized marsupial model for biomedical research. Recent completion of the initial M. domestica genome assembly offers the first opportunity to examine genome-wide phenomena in a marsupial. Using in silico methods, we have mapped 124 conserved microRNAs (miRNAs) to 94 loci in the M. domestica genome. In addition, using RNA pooled from 5 tissues, we cloned 85 miRNAs. Seventy-two of these are conserved miRNAs that we had mapped in silico. The additional 13 are nonconserved candidate miRNAs in 11 loci. Nine of these 13 are also found in the wallaby (Macropus eugenii) genome. Two of the candidate miRNA clones, located on the X chromosome, are part of a cluster containing a total of 24 potential miRNAs spanning more than 100 kb.
Keywords
  • Animals,
  • Base Sequence,
  • Chromosome Mapping,
  • Computational Biology/methods,
  • Conserved Sequence/genetics,
  • Genome,
  • Humans,
  • Macropodidae/genetics,
  • Mice,
  • MicroRNAs/genetics,
  • Molecular Sequence Data,
  • Monodelphis/genetics,
  • Sequence Analysis,
  • RNA
Published Article/Book Citation
The Journal of heredity, 99:1 (2008) pp.66-72.
Citation Information
Eric J. Devor and P. B. Samollow. "In vitro and in silico annotation of conserved and nonconserved microRNAs in the genome of the marsupial Monodelphis domestica" The Journal of heredity Vol. 99 Iss. 1 (2008) p. 66 - 72 ISSN: 1465-7333
Available at: http://works.bepress.com/eric_devor/44/