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Article
Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy
Nucleic Acids Research
  • Eric Henderson, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-1992
DOI
10.1093/nar/20.3.445
Abstract
The atomic force microscope (AFM) was used to image supercooled plasmid DMA deposited on a mica surface in either a hydrated or desiccated state. Hydrated plasmid was precisely cut by the scanning tip at a location determined by the instrument operator. Small pieces of DNA (100–150 nm in length) were excised and deposited adjacent to the dissected plasmid, demonstrating that it is possible to remove and manipulate genomic DNA fragments, unresolvable by light microscopy, from defined chromosomal locations by AFM.
Comments

This article is from Nucleic Acids Research 20 (1992): 445, doi: 10.1093/nar/20.3.445. Posted with permission.

Copyright Owner
Oxford University Press
Language
en
File Format
application/pdf
Citation Information
Eric Henderson. "Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy" Nucleic Acids Research Vol. 20 Iss. 3 (1992) p. 445 - 447
Available at: http://works.bepress.com/eric-henderson/34/