Article
Immunosensing Platforms Using Spontaneously Adsorbed Antibody Fragments on Gold
Analytical Chemistry
Document Type
Article
Disciplines
Publication Version
Published Version
Publication Date
1-1-2000
DOI
10.1021/ac990581e
Abstract
This paper describes the construction and characterization of miniaturized antigenic immunosurfaces composed of spontaneously adsorbed Fab‘-SH fragments on gold. Rabbit Fab‘-SH fragments contain a free sulfhydryl group that forms a thiolate bond with a gold substrate as detailed by X-ray photoelectron spectroscopy. This approach creates surfaces of higher epitope density, a factor critical to the early detection of disease, than surfaces composed of adsorbed whole molecule IgG on gold. The viability and specificity of antigenic Fab‘-SH immunosurfaces is demonstrated using atomic force microscopy and confocal fluorescence microscopy, and possible explanations for the larger epitope density are discussed.
Copyright Owner
American Chemical Society
Copyright Date
2000
Language
en
File Format
application/pdf
Citation Information
Janese C. O'Brien, Vivian W. Jones, Marc D. Porter, Curtis L. Mosher, et al.. "Immunosensing Platforms Using Spontaneously Adsorbed Antibody Fragments on Gold" Analytical Chemistry Vol. 72 Iss. 4 (2000) p. 703 - 710 Available at: http://works.bepress.com/eric-henderson/29/
Reprinted (adapted) with permission from Immunosensing Platforms Using Spontaneously Adsorbed Antibody Fragments on Gold. Janese C. O'Brien,Vivian W. Jones, and, Marc D. Porter, Curtis L. Mosher and Eric Henderson. Analytical Chemistry 2000 72 (4), 703-710. DOI: 10.1021/ac990581e. Copyright 2000 American Chemical Society.