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Monitoring transfected cells without selection agents by using the dual-cassette expression EGFP vectors
Experimental and Molecular Medicine
  • Sang-Gu Kang, Yeungnam University
  • Eunkyung Lee, Yeungnam University
  • Scott Schaus, Iowa State University
  • Eric Henderson, Iowa State University
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Conventional methods of selecting gene transfected cells by toxic agents may yield ambiguous results. It is difficult to determine whether cell death is due to selection agents or gene transfection, owing to the substantial overlap of the time-courses for both effects. Therefore, to determine transfection-induced cell toxicity, the mammalian expression vector pEGFP-N1 (CLONTECH Lab., Palo Alto, CA, USA) has been modified to the dual-cassette expression vectors named pEGFP-Ks by the relocation of its EGFP expression cassette. We have precisely monitored the cells transfected with this vector on our custom culture dishes, thereby bypassing the need for selection agent or fluorescent cell sorting. This is a useful method to screen genes encoding potential toxic or useful proteins without performing undesirable selection agent and also can be used to monitor the transfected cells for various purposes, either the inhibition or proliferation of mammalian cells for applications in biotechnology.

This article is from Experimental and Molecular Medicine 33 (2001): 174, doi: 10.1038/emm.2001.30. Posted with permission.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited
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Sang-Gu Kang, Eunkyung Lee, Scott Schaus and Eric Henderson. "Monitoring transfected cells without selection agents by using the dual-cassette expression EGFP vectors" Experimental and Molecular Medicine Vol. 33 Iss. 3 (2001) p. 174 - 178
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