Article
Chemically Induced Hairpin Formation in DNA Monolayers
Journal of the American Chemical Society
(2002)
Abstract
A naphthyridine dimer that binds specifically to G−G mismatches has been used to induce hairpin formation in oligonucleotides immobilized onto chemically modified gold surfaces. Surface plasmon resonance (SPR) imaging measurements of DNA microarrays were used to demonstrate that binding of the naphthyridine dimer to G−G mismatches within the stem portion of an immobilized 42-mer oligonucleotide could be used to induce hairpin formation that prevented hybridization of DNA complementary to the loop sequence. In addition, the selectivity of the naphthyridine dimer for G−G mismatches was verified through SPR imaging measurements of the hybridization adsorption of an 11-mer oligonucleotide to a four-component DNA array of zero- and single-base mismatch sequences.
Disciplines
Publication Date
May 21, 2002
DOI
10.1021/ja026356n
Publisher Statement
Reprinted (adapted) with permission from Journal of the American Chemical Society, 124(24); 6810-6811. Doi: 10.1021/ja026356n. Copyright 2002 American Chemical Society.
Citation Information
Emily A. Smith, Motoki Kyo, Hiroyuki Kumasawa, Kazuhiko Nakatani, et al.. "Chemically Induced Hairpin Formation in DNA Monolayers" Journal of the American Chemical Society Vol. 124 Iss. 24 (2002) p. 6810 - 6811 Available at: http://works.bepress.com/emily-smith/5/