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Article
Identification and properties of microsatellite markers in tsetse flies Glossina morsitans sensu lato (Diptera: Glossinidae)
Molecular Ecology Notes
  • M. D. Baker, Iowa State University
  • E. S. Krafsur, Iowa State University
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
12-1-2001
DOI
10.1046/j.1471-8278.2001.00087.x
Abstract

Genomic libraries enriched for simple sequence repeats were constructed for Glossina morsitans morsitans, G. m. submorsitans, and G. m. centralis. Sixteen microsatellite markers were isolated from the libraries and evaluated on flies from natural G. m. morsitans populations and other Glossina species in the Morsitans and Palpalis species groups. The primers amplified appropriate sized DNA fragments in the Morsitans and Palpalis groups. In G. morsitans s.l., eight of 12 dinucleotide repeats and four of 12 trinucleotide repeats were polymorphic. The polymorphic loci showed a mean 7.5 ± 4.8 alleles per locus and their mean heterozygosity was 55.8 ± 7.7%.

Comments

This is the peer reviewed version of the following article: Baker, M. D., and E. S. Krafsur. "Identification and properties of microsatellite markers in tsetse flies Glossina morsitans sensu lato (Diptera: Glossinidae)." Molecular ecology notes 1, no. 4 (2001): 234-236, which has been published in final form at doi: 10.1046/j.1471-8278.2001.00087.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Copyright Owner
Blackwell Science Ltd
Language
en
File Format
application/pdf
Citation Information
M. D. Baker and E. S. Krafsur. "Identification and properties of microsatellite markers in tsetse flies Glossina morsitans sensu lato (Diptera: Glossinidae)" Molecular Ecology Notes Vol. 1 Iss. 4 (2001) p. 234 - 236
Available at: http://works.bepress.com/elliot-krafsur/36/