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Article
A cDNA clone from Pisum sativum encoding a low molecular weight heat shock protein.
Nucleic Acids Research (1990)
  • Elizabeth Vierling
Abstract
Pisum sativwn synthesizes multiple low molecular weight (1mw) heat shock proteins (HSPs) when subjected to high temperature stress. We have characterized a cDNA encoding one of the lmw HSPs. A cDNA library was prepared using mRNA isolated from leaves of P.  sativum (cv 'Little Marvel') which had been heat shocked for 120 min at 39°C (1). The library was screened at reduced stringency with a cDNA clone encoding a soybean (Glycine max) lmw  HSP representing the class VI HSP gene family defined by Raschke et al. (2). Pshspl7.7 was identified and found to hybridize to a transcript whose synthesis as  heat inducible  in P. sativum plants. Pshspl7.7 encoded a 157 amino acid, 17.7 kDa protein which is 78.3% identical to Gmhspl7.9-D, as aligned below. Dashes represent identical amino acids, and asterisks indicate a gap inserted to optimize the alignment. This 10 20 30 40 cDNA represents the second complete sequence reported for this class of SPs in plants.
Keywords
  • cDNA,
  • Pisum sativum,
  • heat shock protein
Publication Date
1990
Citation Information
Elizabeth Vierling. "A cDNA clone from Pisum sativum encoding a low molecular weight heat shock protein." Nucleic Acids Research Vol. 18 Iss. 14 (1990) p. 4274
Available at: http://works.bepress.com/elizabeth_vierling/80/