Article
A cDNA clone from Pisum sativum encoding a low molecular weight heat shock protein.
Nucleic Acids Research
(1990)
Abstract
Pisum sativwn synthesizes multiple low molecular weight (1mw) heat shock proteins (HSPs) when subjected to high temperature stress. We have characterized a cDNA encoding one of the lmw HSPs. A cDNA library was prepared using mRNA isolated from leaves of P. sativum (cv 'Little Marvel') which had been heat shocked for 120 min at 39°C (1). The library was screened at reduced stringency with a cDNA clone encoding a soybean (Glycine max) lmw HSP representing the class VI HSP gene family defined by Raschke et al. (2). Pshspl7.7 was identified and found to hybridize to a transcript whose synthesis as heat inducible in P. sativum plants. Pshspl7.7 encoded a 157 amino acid, 17.7 kDa protein which is 78.3% identical to Gmhspl7.9-D, as aligned below. Dashes represent identical amino acids, and asterisks indicate a gap inserted to optimize the alignment. This 10 20 30 40 cDNA represents the second complete sequence reported for this class of SPs in plants.
Keywords
- cDNA,
- Pisum sativum,
- heat shock protein
Disciplines
Publication Date
1990
Citation Information
Elizabeth Vierling. "A cDNA clone from Pisum sativum encoding a low molecular weight heat shock protein." Nucleic Acids Research Vol. 18 Iss. 14 (1990) p. 4274 Available at: http://works.bepress.com/elizabeth_vierling/80/