Article
The use of beta-D-glucanase as a substitute for Novozyme 234 in immunofluorescence and protoplasting
Fungal Genetics Reports
Publication Date
7-25-2000
Abstract
Novozym 234 has been used for many years to prepare protoplasts of Aspergillus nidulans and other fungi for transformation. It has also been very useful in immunofluorescence studies for partially digesting walls of fixed hyphae or germlings to allow antibodies to penetrate into the cytoplasm. In recent years, the availability of Novozym 234 has become problematic, and we have searched for combinations of available enzymes that are suitable for protoplasting and immunofluorescence studies in A. nidulans.
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Creative Commons Attribution-Share Alike 4.0
Citation Information
M Katherine Jung, Yulia Ovechkina, Natalie Prigozhina, C Elizabeth Oakley, et al.. "The use of beta-D-glucanase as a substitute for Novozyme 234 in immunofluorescence and protoplasting" (2000) p. 65 - 66 Available at: http://works.bepress.com/elizabeth_oakley/7/