Distiller’s dried grains with solubles (DDGS) are a common feedstuff in poultry diets, however, digestibility estimates vary with production method and strain (broiler vs layer). The objective of this study was to determine the amino acid digestibility of 34.1% (as fed) high-protein DDGS in straight-run Cobb 500 broiler chickens. Calculated standardized ileal amino acid digestibility (SIAAD) of indispensable amino acids (AA) ranged from 81.20 to 90.41%. The SIAAD of dispensable AA ranged from 16.16 to 89.78%. The digestibility of limiting AA in HiP DDGS was greater than reported digestibility values of the same AA in DDGS. Additional research is needed to further illustrate the effect of DDGS production on the value of feeding high protein (HiP) DDGS in broiler chickens.