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Uptake, Storage and Secretion of 5-hydroxytryptamine and Its Amino Acid Precursor by Dispersed Rat Pancreas Acinar Cells
The Journal of Physiology (1983)
  • Ligia Stern, McGill University
  • A. Tenenhouse, McGill University
  • E. W.-T. Yu, McGill University
Rat pancreas acinar cells contain 5-hydroxytryptamine (5-HT; 10.86 +/- 2.52 ng/i.u. amylase), all of which can be accounted for by the 5-HT recovered from the zymogen granule fraction of these cells (10.70 +/- 3.06 ng/i.u. amylase). When incubated with [14C]5-HT dispersed acinar cells take up the amine and concentrate it in zymogen granules. These cells will also take up [14C]5-HTP (5-hydroxytryptophan), decarboxylate it and store the [14C]5-HT so produced in zymogen granules. 5-HTP itself is not taken up by the granules. 5-HT is incorporated into zymogen granules early in their formation; no amine is accumulated by mature zymogen granules and the amine within the mature granule is not exchangeable with extragranular amine. When dispersed acinar cells pre-labelled with [14C]5-HT and [3H]leucine are stimulated with caerulein, there is a synchronous increase in amylase activity and secretion of [14C]5-HT and [3H]protein; the ratios of [3H]protein/[14C]5-HT in zymogen granules and in the secretory products are identical. Pancreas acinar cells take up L-DOPA, decarboxylate it and store the dopamine produced in zymogen granules but the dopamine is not retained by the granules (t1/2 approximately equal to 90 min) and dopamine secretion from cells exposed to caerulein could not be demonstrated. It is concluded that 5-HT is a normal component of rat pancreas acinar cell zymogen granule. The granular amine has a turnover rate similar to that of granular protein and is released when the cells are stimulated to secrete protein. All the 5-HT released from the cell originates in zymogen granules.
  • 5-Hydroxytryptophan,
  • Amylases,
  • Animals,
  • Caerulein,
  • Cytoplasmic Granules,
  • Dopamine,
  • Enzyme Precursors,
  • Pancreas,
  • Proteins,
  • Rats,
  • Rats,
  • Inbred Strains,
  • Serotonin,
  • Subcellular Fractions,
  • Time Factors
Publication Date
July 1, 1983
Publisher Statement
Dr. Edward Yu is currently a faculty member at The University of Western Ontario.
Citation Information
Ligia Stern, A. Tenenhouse and E. W.-T. Yu. "Uptake, Storage and Secretion of 5-hydroxytryptamine and Its Amino Acid Precursor by Dispersed Rat Pancreas Acinar Cells" The Journal of Physiology Vol. 340 Iss. 1 (1983)
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