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Article
Ultrastructure of Blood-Brain Barrier and Blood-Spinal Cord Barrier in SOD1 Mice Modeling ALS
Brain Research
  • S Garbuzova-Davis, University of South Florida
  • Edward M Haller, University of South Florida
  • Samuel Saporta, University of South Florida
  • Irina Kolomey, University of South Florida
  • Santo V. Nicosia, University of South Florida
  • Paul R. Sanberg, University of South Florida
Document Type
Article
Publication Date
7-9-2007
Keywords
  • Blood–brain barrier,
  • Blood–spinal cord barrier,
  • Ultrastructure,
  • G93A SOD1 mice,
  • Brainstem,
  • Spinal cord,
  • Endothelium,
  • Basement membrane,
  • Mitochondria,
  • Motor neuron
Digital Object Identifier (DOI)
https://doi.org/10.1016/j.brainres.2007.04.044
Abstract

The purpose of this study was to determine the ultrastructure of the blood–brain barrier (BBB) and blood–spinal cord barrier (BSCB) in G93A SOD1 mice modeling ALS at different stages of disease. Electron microscope examination of brainstem, cervical and lumbar spinal cords was performed in ALS mice at early and late stages of disease. Our results show disorganized mitochondrial cristae and degenerating mitochondria in endothelial cells and neuropil, swollen astrocyte foot processes, swollen and degenerating capillary endothelial cells, astrocytes and motor neurons and extensive extracellular edema. In spite of progressive extracellular edema in neural tissue, capillary endothelial cell tight junctions appeared to remain intact in early and late symptomatic animals. Results show that disruption of BBB and BSCB was evident in areas of motor neuron degeneration in G93A mice at both early and late stages of disease. Capillary rupture was observed in brainstem in early symptomatic G93A mice. Capillary ultrastructure revealed that endothelial cell membrane and/or basement membrane damage occurred, followed by vascular leakage

Citation / Publisher Attribution

Brain Research, v. 1157, p. 126-137

Citation Information
S Garbuzova-Davis, Edward M Haller, Samuel Saporta, Irina Kolomey, et al.. "Ultrastructure of Blood-Brain Barrier and Blood-Spinal Cord Barrier in SOD1 Mice Modeling ALS" Brain Research Vol. 1157 (2007) p. 126 - 137
Available at: http://works.bepress.com/edward_haller/30/