A new species of arboreal Eleutherodactylus is described and assigned to the Eleutherodactylus unistrigatus Group from the Amazonian lowlands of northern Departamento de Cusco, Peru. The new species was found on vegetation 150–800 cm above ground, has a SVL up to 21.9 mm, and is distinguished from other species of the genus by having a green dorsum with white spots; a pointed, brown snout; and by lacking a tympanic membrane and annulus. Males lack vocal slits and nuptial pads; females are unknown.