The depth jump (DJ) is commonly used to evaluate athletic ability, and has further application in rehabilitation and injury prevention. There is limited research exploring sex-based differences in DJ ground reaction force (GRF) measures. This study aimed to evaluate for sex-based differences in DJ GRF measures and determine sample size thresholds for binary classification of sex. Forty-seven participants from mixed-sex samples of NCAA athletes and young adults performed DJs from various drop heights. Force platform dynamometry and 2-dimensional videography were used to estimate GRF measures. Three-way mixed analysis of variance was used to evaluate main effects and interactions. Receiver operating characteristic (ROC) curve analysis was used to evaluate the combined sensitivity and specificity of dependent measures to sex. Results revealed that reactive strength index scores and rebound jump heights were greater in males than females (p < 0.001). Additionally, young adult females showed greater peak force reduction than young adult males (p = 0.002). ROC curve analysis revealed mixed results that appeared to be influenced by population characteristics and drop height. In conclusion, sex-based differences in DJ performance were observed, and the results of this study provide direction for future DJ investigations.