Article
Biomimetic Antigenic Nanoparticles Elicit Controlled Protective Immune Response to Influenza
ACS Nano
(2013)
Abstract
Here we present a biomimetic strategy toward nanoparticle design for controlled immune response through encapsulation of conserved internal influenza proteins on the interior of virus-like particles (VLPs) to direct CD8+ cytotoxic T cell protection. Programmed encapsulation and sequestration of the conserved nucleoprotein (NP) from influenza on the interior of a VLP, derived from the bacteriophage P22, results in a vaccine that provides multistrain protection against 100 times lethal doses of influenza in an NP specific CD8+ T cell-dependent manner. VLP assembly and encapsulation of the immunogenic NP cargo protein is the result of a genetically programmed self-assembly making this strategy amendable to the quick production of vaccines to rapidly emerging pathogens. Addition of adjuvants or targeting molecules were not required for eliciting the protective response.
Keywords
- virus-like particle,
- VLP,
- P22,
- nucleoprotein,
- influenza,
- CD8,
- biomimetic
Disciplines
Publication Date
2013
DOI
10.1021/nn4006544
Publisher Statement
First published in ACS Nano.
Citation Information
Patterson, D. P., Rynda-Apple, A., Harmsen, A. L., Harmsen, A. G., & Douglas, T. (2013). Biomimetic Antigenic Nanoparticles Elicit Controlled Protective Immune Response to Influenza. ACS Nano, 7(4), 3036–3044.