Article
Encapsulation of an Enzyme Cascade within the Bacteriophage P22 Virus-Like Particle
ACS Chemical Biology
(2014)
Abstract
Developing methods for investigating coupled enzyme systems under conditions that mimic the cellular environment remains a significant challenge. Here we describe a biomimetic approach for constructing densely packed and confined multienzyme systems through the co-encapsulation of 2 and 3 enzymes within a virus-like particle (VLP) that perform a coupled cascade of reactions, creating a synthetic metabolon. Enzymes are efficiently encapsulated in vivo with known stoichiometries, and the kinetic parameters of the individual and coupled activities are characterized. From the results we develop and validate a mathematical model for predicting the expected kinetics for coupled reactions under co-localized conditions.
Disciplines
Publication Date
2014
DOI
10.1021/cb4006529
Publisher Statement
First published in ACS Chemical Biology.
Citation Information
Patterson, D. P., Schwarz, B., Waters, R. S., Gedeon, T., & Douglas, T. (2014). Encapsulation of an Enzyme Cascade within the Bacteriophage P22 Virus-Like Particle. ACS Chemical Biology, 9(2), 359–365.