Article
Design of a VLP-nanovehicle for CYP450 enzymatic activity delivery
Journal of Nanobiotechnology
(2015)
Abstract
Background
The intracellular delivery of enzymes for therapeutic use has a promising future for the treatment of several diseases such as genetic disorders and cancer. Virus-like particles offer an interesting platform for enzymatic delivery to targeted cells because of their great cargo capacity and the enhancement of the biocatalyst stability towards several factors important in the practical application of these nanoparticles.
Results
We have designed a nano-bioreactor based on the encapsulation of a cytochrome P450 (CYP) inside the capsid derived from the bacteriophage P22. An enhanced peroxigenase, CYPBM3, was selected as a model enzyme because of its potential in enzyme prodrug therapy. A total of 109 enzymes per capsid were encapsulated with a 70 % retention of activity for cytochromes with the correct incorporation of the heme cofactor. Upon encapsulation, the stability of the enzyme towards protease degradation and acidic pH was increased. Cytochrome P450 activity was delivered into Human cervix carcinoma cells via transfecting P22-CYP nanoparticles with lipofectamine.
Conclusion
This work provides a clear demonstration of the potential of biocatalytic virus-like particles as medical relevant enzymatic delivery vehicles for clinical applications.
Keywords
- Enzymatic delivery,
- Cytochrome P450,
- Virus-like particles,
- Nanobioreactor,
- Enzyme produg therapy
Disciplines
Publication Date
October 9, 2015
DOI
10.1186/s12951-015-0127-z
Publisher Statement
First published in Journal of Nanobiotechnology.
Citation Information
Sánchez‑Sánchez, L., Tapia‑Moreno, A., Juarez‑Moreno, K., Patterson, D. P., Cadena‑Nava, R. D., Douglas, T., & Vazquez‑Duhalt, R. (2015). Design of a VLP‑nanovehicle for CYP450 enzymatic activity delivery. Journal of Nanobiotechnology, 13(33).