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Selected Works of Dustin Patterson
Article
Viruslike Particles Encapsidating Respiratory Syncytial Virus M and M2 Proteins Induce Robust T Cell Responses
ACS Biomaterials Science & Engineering (2016)
  • Benjamin Schwarz, Indiana University Bloomington
  • Kaitlyn M. Morabito, Georgetown University
  • Kaitlyn M. Morabito, National Institutes of Health
  • Tracy J. Ruckwardt, National Institutes of Health
  • Dustin P. Patterson
  • John Avera, Indiana University Bloomington
  • Heini M. Miettinen, Montana State University
  • Barney S. Graham, National Institutes of Health
  • Trevor Douglas, Indiana University Bloomington
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Abstract
Subunit vaccines provide a safe, focused alternative to conventional vaccines. However, these vaccines often require significant adjuvants and are particularly hard to target toward cytotoxic T lymphocyte (CTL) immunity. Viruslike particles (VLPs) provide biomaterial scaffolds with pathogen-like polyvalent structures making them useful platforms for biomimetic antigen delivery to the immune system. Encapsidation of antigens within VLPs has been shown to enhance antigen availability for CD8 T cell responses. Here, we examine the potential to generate complex responses to multiple subunit antigens localized within the same VLP particle. Two proteins of respiratory syncytial virus (RSV) with well-characterized CD8 T cell responses, the matrix (M) and matrix 2 (M2) proteins, were successfully coencapsidated within the P22 VLP. Upon intranasal administration in mice, the particles stimulated CD8 T cell memory responses against both antigens. In addition, vaccination elicited tissue-resident T cell populations. Upon subsequent RSV challenge, P22-M/M2-treated mice displayed significantly reduced lung viral titers. This demonstrates the utility of the P22 VLP in directing immune responses to multiple encapsidated viral antigens, demonstrating the potential of this technology to facilitate immunity to multiple targets simultaneously.
Keywords
  • subunit vaccine viruslike particle respiratory syncytial virus CD8+ T cell immunity tissue-resident memory T cells
Disciplines
Publication Date
December 12, 2016
DOI
10.1021/acsbiomaterials.6b00532
Citation Information
Benjamin Schwarz, Kaitlyn M. Morabito, Kaitlyn M. Morabito, Tracy J. Ruckwardt, et al.. "Viruslike Particles Encapsidating Respiratory Syncytial Virus M and M2 Proteins Induce Robust T Cell Responses" ACS Biomaterials Science & Engineering Vol. 2 Iss. 12 (2016) p. 2324 - 2332
Available at: http://works.bepress.com/dustin-patterson/21/