Article
Self-assembling biomolecular catalysts for hydrogen production
Nature Chemistry
(2015)
Abstract
The chemistry of highly evolved protein-based compartments has inspired the design of new catalytically active materials that self-assemble from biological components. A frontier of this biodesign is the potential to contribute new catalytic systems for the production of sustainable fuels, such as hydrogen. Here, we show the encapsulation and protection of an active hydrogen-producing and oxygen-tolerant [NiFe]-hydrogenase, sequestered within the capsid of the bacteriophage P22 through directed self-assembly. We co-opted Escherichia coli for biomolecular synthesis and assembly of this nanomaterial by expressing and maturing the EcHyd-1 hydrogenase prior to expression of the P22 coat protein, which subsequently self assembles. By probing the infrared spectroscopic signatures and catalytic activity of the engineered material, we demonstrate that the capsid provides stability and protection to the hydrogenase cargo. These results illustrate how combining biological function with directed supramolecular self-assembly can be used to create new materials for sustainable catalysis.
Keywords
- Biocatalysis,
- Bioinspired materials,
- Nanobiotechnology,
- Self-assembly
Disciplines
Publication Date
December 21, 2015
DOI
10.1038/nchem.2416
Publisher Statement
First published in Nature Chemistry.
Citation Information
Jordan, P., Patterson, D. P., Kendall, S., Edwards, E. J., Miettinen, H. M., Basu, G., … Douglas, T. (2016). Self-assembling biomolecular catalysts for hydrogen production. Nature Chemistry, 8, 179–185.