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Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
Nature Methods
  • Jeffry D. Sander, Massachusetts General Hospital
  • Elizabeth J. Dahlborg, Massachusetts General Hospital
  • Matthew J. Goodwin, Massachusetts General Hospital
  • Lindsay Cade, Massachusetts General Hospital
  • Feng Zhang, University of Minnesota - Twin Cities
  • Daniel Cifuentes, Yale University School of Medicine
  • Shaun J. Curtin, University of Minnesota - Twin Cities
  • Jessica S. Blackburn, Massachusetts General Hospital
  • Stacey Thibodeau-Beganny, Massachusetts General Hospital
  • Yiping Qi, University of Minnesota - Twin Cities
  • Christopher J. Pierick, University of Minnesota - Twin Cities
  • Ellen Hoffman, Yale University School of Medicine
  • Morgan L. Maeder, Massachusetts General Hospital
  • Cyd Khayter, Massachusetts General Hospital
  • Deepak Reyon, Iowa State University
  • Drena Dobbs, Iowa State University
  • David M. Langenau, Massachusetts General Hospital
  • Robert M. Stupar, University of Minnesota - Twin Cities
  • Antonio J. Giraldez, Yale University School of Medicine
  • Daniel F. Voytas, University of Minnesota - Twin Cities
  • Randall T. Peterson, Massachusetts General Hospital
  • Jing-Ruey J. Yeh, Massachusetts General Hospital
  • J. Keith Joung, Massachusetts General Hospital
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
1-1-2011
DOI
10.1038/nmeth.1542
Abstract
Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.
Comments

This is a manuscript of an article from Nature Methods 8 (2011): 67, doi: 10.1038/nmeth.1542. Posted with permission.

Copyright Owner
Nature American, Inc.
Language
en
File Format
application/pdf
Citation Information
Jeffry D. Sander, Elizabeth J. Dahlborg, Matthew J. Goodwin, Lindsay Cade, et al.. "Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)" Nature Methods Vol. 8 Iss. 1 (2011) p. 67 - 69
Available at: http://works.bepress.com/drena-dobbs/36/