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Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool
Nucleic Acids Research
  • Jeffry D. Sander, Iowa State University
  • Peter Zaback, Iowa State University
  • J. Keith Joung, Massachusetts General Hospital
  • Daniel F. Voytas, Iowa State University
  • Drena Dobbs, Iowa State University
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Zinc Finger Targeter (ZiFiT) is a simple and intuitive web-based tool that facilitates the design of zinc finger proteins (ZFPs) that can bind to specific DNA sequences. The current version of ZiFiT is based on a widely employed method of ZFP design, the ‘modular assembly’ approach, in which pre-existing individual zinc fingers are linked together to recognize desired target DNA sequences. Several research groups have described experimentally characterized zinc finger modules that bind many of the 64 possible DNA triplets. ZiFiT leverages the combined capabilities of three of the largest and best characterized module archives by enabling users to select fingers from any of these sets. ZiFiT searches a query DNA sequence for target sites for which a ZFP can be designed using modules available in one or more of the three archives. In addition, ZiFiT output facilitates identification of specific zinc finger modules that are publicly available from the Zinc Finger Consortium. ZiFiT is freely available at

This article is from Nucleic Acids Research 35 (2007): W599, doi: 10.1093/nar/gkm349. Posted with permission.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Jeffry D. Sander, Peter Zaback, J. Keith Joung, Daniel F. Voytas, et al.. "Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool" Nucleic Acids Research Vol. 35 (2007) p. W599 - W605
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