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Article
Visualization of circular DNA molecules labeled with colloidal gold spheres using atomic force microscopy
Journal of Vacuum Science & Technology A
  • Wen-Ling Shaiu, Iowa State University
  • James Vesenka, Iowa State University
  • Daniel Jondle, Iowa State University
  • Eric Henderson, Iowa State University
  • Drena D. Larson, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-1993
DOI
10.1116/1.578311
Abstract

We have imaged gold‐labeled DNA molecules with the atomic force microscope(AFM). Circular plasmid DNA was labeled at internal positions by nick‐translation using biotinylated dUTP. For visualization, the biotinylated DNA was linked to streptavidin‐coated colloidal gold spheres (nominally 5 nm diam) prior to AFM imaging. Reproducible images of the labeled DNA were obtained both in dry air and under propanol. Height measurements of the DNA and colloidal gold made under both conditions are presented. The stability of the DNA‐streptavidin colloidal gold complexes observed even under propanol suggests that this labeling procedure could be exploited to map regions of interest in chromosomal DNA.

Comments

This article is from Journal of Vacuum Science & Technology A 11 (1993): 820, doi: 10.1116/1.578311. Posted with permission.

Copyright Owner
American Vacuum Society
Language
en
File Format
application/pdf
Citation Information
Wen-Ling Shaiu, James Vesenka, Daniel Jondle, Eric Henderson, et al.. "Visualization of circular DNA molecules labeled with colloidal gold spheres using atomic force microscopy" Journal of Vacuum Science & Technology A Vol. 11 Iss. 4 (1993) p. 820 - 823
Available at: http://works.bepress.com/drena-dobbs/26/