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Atomic force microscopy of oriented linear DNA molecules labeled with 5nm gold spheres
Nucleic Acids Research
  • Wen-Ling Shaiu, Iowa State University
  • Drena D. Larson, Iowa State University
  • James Vesenka, Iowa State University
  • Eric Henderson, Iowa State University
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The atomic force microscope (AFM;1) can image DNA and RNA in air and under solutions at resolution comparable to that obtained by electron microscopy (EM) (2–7). We have developed a method for depositing and imaging linear DNA molecules to which 5nm gold spheres have been attached. The gold spheres facilitate orientation of the DNA molecules on the mica surface to which they are adsorbed and are potentially useful as internal height standards and as high resolution gene or sequence specific tags. We show that by modulating their adhesion to the mica surface, the gold spheres can be moved with some degree of control with the scanning tip.

This article is from Nucleic Acids Research 21 (1993): 99, doi: 10.1093/nar/21.1.99. Posted with permission.

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Oxford University Press
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Wen-Ling Shaiu, Drena D. Larson, James Vesenka and Eric Henderson. "Atomic force microscopy of oriented linear DNA molecules labeled with 5nm gold spheres" Nucleic Acids Research Vol. 21 Iss. 1 (1993) p. 99 - 103
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