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Article
Microfluidics Devices Integrating Microcavity Surface‐Plasmon‐Resonance Biosensors: Glucose Oxidase Binding Activity Detection
Analytical Chemistry (2010)
  • Dragos Amarie, Indiana University - Bloomington
  • Abdelkrim Alileche, Indiana University - Bloomington
  • Bogdan Dragnea, Indiana University - Bloomington
  • James A. Glazier, Indiana University - Bloomington
Abstract
We have developed miniature (≈1 μm diameter) microcavity surface-plasmon-resonance sensors (MSPRS), integrated them with microfluidics and tested their sensitivity to refractive-index changes. We tested their biosensing capability by distinguishing the interaction of glucose oxidase (Mr 160 kDa) with its natural substrate (β-D-glucose, Mr 180 Da) from its interactions with non-specific substrates (L-glucose, D-mannose and 2-deoxy-D-glucose). We ran the identical protocol we had used with the MSPRS on a Biacore 3000 instrument using their bare gold chip. Only the MSPRS was able to detect β-D-glucose binding to glucose oxidase. Each MSPRS can detect the binding to its surface of fewer than 35,000 glucose-oxidase molecules (representing 9.6 fg or 60 zmol of protein), about 106 times fewer than classical surface-plasmon-resonance biosensors. 
Keywords
  • Microcavity surface-plasmon-resonance sensors,
  • MSPRS,
  • Microfluidics,
  • Glucose oxidase
Disciplines
Publication Date
January 1, 2010
DOI
10.1021/ac902038d
Publisher Statement
This is an Accepted Author Manuscript obtained from PMC. The publisher's final edited version of this article is available at AnalyticalChemistry. 
Citation Information
Dragos Amarie, Abdelkrim Alileche, Bogdan Dragnea and James A. Glazier. "Microfluidics Devices Integrating Microcavity Surface‐Plasmon‐Resonance Biosensors: Glucose Oxidase Binding Activity Detection" Analytical Chemistry Vol. 82 Iss. 1 (2010) p. 343 - 352
doi:10.1021/ac902038d
source:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2824604/
Available at: http://works.bepress.com/dragos-amarie/4/