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Microfluidics Devices Integrating Microcavity Surface‐Plasmon‐Resonance Biosensors: Glucose Oxidase Binding Activity Detection
Analytical Chemistry (2010)
  • Dragos Amarie, Indiana University - Bloomington
  • Abdelkrim Alileche, Indiana University - Bloomington
  • Bogdan Dragnea, Indiana University - Bloomington
  • James A. Glazier, Indiana University - Bloomington
We have developed miniature (≈1 μm diameter) microcavity surface-plasmon-resonance sensors (MSPRS), integrated them with microfluidics and tested their sensitivity to refractive-index changes. We tested their biosensing capability by distinguishing the interaction of glucose oxidase (Mr 160 kDa) with its natural substrate (β-D-glucose, Mr 180 Da) from its interactions with non-specific substrates (L-glucose, D-mannose and 2-deoxy-D-glucose). We ran the identical protocol we had used with the MSPRS on a Biacore 3000 instrument using their bare gold chip. Only the MSPRS was able to detect β-D-glucose binding to glucose oxidase. Each MSPRS can detect the binding to its surface of fewer than 35,000 glucose-oxidase molecules (representing 9.6 fg or 60 zmol of protein), about 106 times fewer than classical surface-plasmon-resonance biosensors. 
  • Microcavity surface-plasmon-resonance sensors,
  • MSPRS,
  • Microfluidics,
  • Glucose oxidase
Publication Date
January 1, 2010
Publisher Statement
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © 2010 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see Analytical Chemistry. This Accepted Author Manuscript was obtained from PMC.
Citation Information
Dragos Amarie, Abdelkrim Alileche, Bogdan Dragnea and James A. Glazier. "Microfluidics Devices Integrating Microcavity Surface‐Plasmon‐Resonance Biosensors: Glucose Oxidase Binding Activity Detection" Analytical Chemistry Vol. 82 Iss. 1 (2010) p. 343 - 352 ISSN: 1520-6882
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