Article
Preparative LC and non-porous silica chromatography as useful tools in large scale dendrimer synthesis
Journal of Liquid Chromatography and Related Technologies
(2002)
Abstract
The convergent synthesis of poly(benzyl ether) dendrimers was published by Frechet et al. However, the coupling of the lower generation bromide with the 3,5-dihydroxy benzyl alcohol (monomer) involved the use of triphenylphosphine. The purification of higher generation dendrimers was difficult because triphenylphosphine was not easily separated from the desired product. A different approach was studied in our laboratory. A mesylate intermediate was developed, followed by the coupling of the monomer. Dendrimers generated from this process were separated using preparative LC chromatography. Non-porous silica (NPS) chromatography methods were developed to monitor the mesylation. and coupling reactions and to confirm the purity of the preparative chromatography fractions.
Keywords
- Dendrimer synthesis,
- Non-porous silica,
- Preparative LC
Disciplines
Publication Date
February 6, 2002
DOI
10.1081/JLC-120004757
Citation Information
Helen Chao, Charles J. Shaw and James E Hanson. "Preparative LC and non-porous silica chromatography as useful tools in large scale dendrimer synthesis" Journal of Liquid Chromatography and Related Technologies Vol. 25 Iss. 9 (2002) p. 1421 - 1430 Available at: http://works.bepress.com/dr_james_hanson/22/