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Article
Separation of Phosvitin from Egg Yolk without Using Organic Solvents
Asian-Australasian Journal of Animal Sciences
  • Samooel Jung, Seoul National University
  • Dong U. Ahn, Iowa State University
  • Ki Chang Nam, Sunchon National University
  • Hyun Joo Kim, Seoul National University
  • Cheorun Jo, Seoul National University
Document Type
Article
Publication Version
Published Version
Publication Date
11-1-2013
DOI
10.5713/ajas.2013.13263
Abstract

The objective of this study was to develop a new method to separate phosvitin from egg yolk without using organic solvents. Phosvitin was extracted from yolk granules using 10% NaCl or 10% (NH4)2SO4 (final concentration) and then treated with heat to precipitate the lipoproteins from the extracted solution. The optimal pH for the phosvitin extraction from yolk granules was determined, and the iron-binding ability of the extracted phosvitin (final product) was tested. Adding 10% (NH4)2SO4 disrupted the granules, and the subsequent thermal treatment at 90°C for 1 h precipitated low density and high density lipoproteins, which enabled separation of phosvitin by centrifugation. The phosvitin concentration in the extract was significantly higher when the pH of the solution was adjusted to pH ≥9. The purity and recovery rate of phosvitin at the end of the separation process were approximately 78% and 56%, respectively. The separated phosvitin was confirmed to have ferrous and ferric iron binding ability. The advantages of this new method compared with the traditional methods include no organic solvents and high-priced equipment are needed for the separation. Also, this method is more environment and consumer friendly than that of the traditional methods.

Comments

This article is from Asian-Australasian Journal of Animal Sciences 26 (2013): 1622, doi:10.5713/ajas.2013.13263. Posted with permission.

Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright Owner
Samooel Jung, et al.
Language
en
Date Available
2015-05-13
File Format
application/pdf
Citation Information
Samooel Jung, Dong U. Ahn, Ki Chang Nam, Hyun Joo Kim, et al.. "Separation of Phosvitin from Egg Yolk without Using Organic Solvents" Asian-Australasian Journal of Animal Sciences Vol. 26 Iss. 11 (2013) p. 1622 - 1629
Available at: http://works.bepress.com/dong_ahn/70/