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Article
RNA polymerase activity of Amsacta moorei entomopox virions
Intervirology
  • W. J. McCarthy
  • C. F. Neser
  • Donald W. Roberts, Utah State University
Document Type
Article
Publication Date
1-1-1975
DOI
10.1159/000149882
Disciplines
Abstract
Optimum conditions for in vitro RNA synthesis by the entomopoxvirus from Amsacta moorei, except for a temperature optimum of 26 degrees, were similar to those reported for vaccinia. Incorporation of 3H-ATP in the presence of CTP, GTP and UTP was significantly inhibited by actinomycin D; incorporation of 3H-ATP alone was not. The products formed by incorporation of 3H-ATP alone or with the three other nucleotides contained polyadenylic acid sequences. The sedimentation coefficient of the 3H-ATP-labeled product formed in the presence of CTP, GTP and UTP was reduced from 8-23S to 3-5S after RNase treatment. The product formed from 3H-ATP alone had an apparent sedimentation value of 3-5S.
Citation Information
McCarthy, W.J., C.F. Neser and D.W. Roberts. 1975. RNA polymerase activity of Amsacta moorei entomopox virions. Intervirology 5: 69-75.