During early embryonic development, cranialneuralcrest cells emerge from the developing mid- and hindbrain. While numerous studies have focused on integrin involvement in trunk neuralcrest cell migration, comparatively little is known about mechanisms of cranialneuralcrest cell migration. We show that fibronectin, but not laminin, vitronectin, or type I collagen can supportcranialneuralcrest cell migration and segmentation in vitro. These behaviors require both the RGD and “synergy” sites located within the central cell-binding domain of fibronectin. While these two sites are sufficient for cranialneuralcrest cell migration, we find that the second Heparin-binding domain of fibronectin can provide additional support for cranialneuralcrest cell migration in vitro. Finally, using a function blocking monoclonal antibody, we show that cranialneuralcrest cell migration on fibronectin requires the integrin α5β1.
Available at: http://works.bepress.com/dominique_alfandari/7/