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A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories
Journal of Chemical Education
  • Reza Razeghifard, Nova Southeastern University
  • Catherine Chiafair, Nova Southeastern University
  • Dimitri Giarikos, Nova Southeastern University
Publication Date
  • Upper-Division Undergraduate,
  • Biochemistry,
  • Laboratory Instruction,
  • Collaborative/Cooperative Learning,
  • Bioorganic Chemistry,
  • Qualitative Analysis,
  • Enzymes,
  • Gas Chromatography,
  • Mass Spectrometry,
  • Quantitative Analysis
A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene (BHT) by liver microsomes using gas chromatography–mass spectrometry (GC–MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC–MS analysis, data interpretation of complex mixtures, and collaborative work.

©2013 The American Chemical Society and Division of Chemical Education, Inc.

Citation Information
Reza Razeghifard, Catherine Chiafair and Dimitri Giarikos. "A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories" Journal of Chemical Education Vol. 91 Iss. 1 (2014) p. 141 - 144 ISSN: 0021-9584
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