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HLA Class I (ABC) Upregulation on Peripheral Blood CD3+/CD8+ T Lymphocyte Surface is a Potential Predictor of Acute Rejection in Renal Transplantation
Transplantation
  • Jun Tian, Department of Renal Transplantation, Qilu Hospital, Shandong University, Jinan, Shandong People's Republic of China;
  • Wen-Feng Shi, Department of Renal Transplantation, Qilu Hospital, Shandong University, Jinan, Shandong People's Republic of China
  • Lu-Wei Zhang, Department of Renal Transplantation, Qilu Hospital, Shandong University, Jinan, Shandong People's Republic of China
  • Nan Lu, Institute of Diagnostics, Shandong University, Jinan Shandong, People's Republic of China
  • Xian-Quan Cui, Department of Renal Transplantation, Qilu Hospital, Shandong University, Jinan, Shandong People's Republic of China
  • Wan-Hua Xian, Department of Renal Transplantation, Qilu Hospital, Shandong University, Jinan, Shandong People's Republic of China
  • Huai-Bin Sun, Department of Renal Transplantation, Qilu Hospital, Shandong University, Jinan, Shandong People's Republic of China
  • En-Gang Li, Department of Renal Transplantation, Qilu Hospital, Shandong University, Jinan, Shandong People's Republic of China
  • Li-Na Geng, Department of Renal Transplantation, Qilu Hospital, Shandong University, Jinan, Shandong People's Republic of China
  • Dianzheng Zhang, Philadelphia College of Osteopathic Medicine
  • Xiong Zou, Department of Clinical Laboratory, Qilu Hospital, Shandong University, Shandong, People's Republic of China
Document Type
Article
Publication Date
12-27-2009
Abstract
BACKGROUND: Renal transplantation is currently the prevalent therapy for most patients with end-stage renal disease. No clinical markers for such rejection have been universally accepted. We aimed to investigate the possibility of use of human leukocyte antigen (HLA) class I (ABC) on peripheral blood CD3+/CD8+ T lymphocytes as a marker of acute rejection. METHODS: For recipients undergoing renal transplantation from September 2007 to November 2008, peripheral blood samples were obtained pretransplantation and at days 3 and 7 posttransplantation when the patients were still hospitalized and at weeks 2 and 3 and months 1, 2, 3, and 6 posttransplantation. For patients with fever, lumbodynia, gross hematuria, or oliguria after transplantation, blood samples were collected immediately before and at days 3 and 7 after the administration of anti-inflammatory regents. The level of HLA class I (ABC) on peripheral-blood CD3+/CD8+ T lymphocytes was measured on flow cytometry. RESULTS: For the 79 transplant recipients, the level of HLA class I (ABC) on peripheral-blood CD3+/CD8+ T lymphocytes was consistently elevated during the first 3 weeks after transplantation, declined gradually to pretransplantation levels, then tapered off and remained stable. Patients experiencing acute rejection (AR) or not after transplantation did not differ in level of HLA class I (ABC) up to 6-month follow-up, except at days 14 and 21 after transplantation, when the level was higher for patients experiencing AR (P<0.01). CONCLUSIONS: Upregulation of HLA class I (ABC) on peripheral-blood CD3+/CD8+ T lymphocytes could be used as an accurate and reliable predictor of AR after renal transplantation.
PubMed ID
20029336
Comments

This article was published in Transplantation, Volume 88, Issue 12, December 27, 2009, pages 1393-1397.

The published version is available at http://dx.doi.org/10.1097/TP.0b013e3181bc5c94

Copyright © 2000-2012 The Transplantation Society

Citation Information
Jun Tian, Wen-Feng Shi, Lu-Wei Zhang, Nan Lu, et al.. "HLA Class I (ABC) Upregulation on Peripheral Blood CD3+/CD8+ T Lymphocyte Surface is a Potential Predictor of Acute Rejection in Renal Transplantation" Transplantation Vol. 88 Iss. 12 (2009) p. 1393 - 1397
Available at: http://works.bepress.com/dianzheng_zhang/8/